دانشگاه آزاد اسلامی واحد تهران جنوب

صفحه اصلی / اولین کنفرانس ملی شیمی، نانو مواد، پلیمر- چالش ها و کاربردها

Trace monitoring of drug in human plasma utilizing magnetic solid phase extraction based carbonized cotton fabric/Fe3O4 in combination with HPLC

نویسندگان :

Yasaman Sanaei¹ Mohsen Zeeb²

1- دانشگاه آزاد اسلامی واحد تهران جنوب 2- دانشگاه آزاد اسلامی واحد تهران جنوب

کلمات کلیدی :

Carbonized cotton fabric،Magnetic solid phase extraction،High-performance liquid chromatography،Fe3O4

چکیده :

Carbonized cotton Fabric (CCF) was fabricated as sorbent for magnetic solid phase extraction (SPE) of drug in human plasma, which was combined with high performance liquid chromatography ultraviolent detection (HPLC–UV). The Carbonized cotton Fabric was syntesised by using cotton fabric and modified by Fe3O4 because of its extremely high magnetic properties and allow the sorbent to be easily divided from the sample solution by using an external magnet. Sorbent characterization was carried out utilizing X-ray diffraction (XRD), Fourier transform-infrared (FT-IR) spectroscopy and Field-emission scanning electron microscopy (FE-SEM). Important analytical parameters such as pH, amount of sorbent, desorption condition and ultrasonic bath time that effect the developed magnetic solid phase extraction method were investigated and optimum parameters were detected. Carbonized cotton Fabric / Fe3O4 was used as sorbent for the determination and preconcentration of drug in human plasma and some pharmacokinetic data were comprehensively examined.